412-79v9 - EC-Council Certified Security Analyst (ECSA V9) 2020 Robotemotion

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ECCouncil 412-79v9 : EC-Council Certified Security Analyst (ECSA V9) Exam

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Test Number : 412-79v9
Test title : EC-Council Certified Security Analyst (ECSA V9)
Vendor title : ECCouncil
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412-79v9 test Format | 412-79v9 Course Contents | 412-79v9 Course contour | 412-79v9 test Syllabus | 412-79v9 test Objectives

Credit Towards Certification: ECSA v10
Number of Questions: 150
Passing Score: 70%
Test Duration: 4 Hours

Computer Network Fundamentals
Network Security Controls and Devices
Windows and Linux Security
Web Application and Web Server Architecture and Operations
Web Application Security Mechanisms
Information Security Attacks
Information Security Standards
Penetration Testing Process and Methodologies & Benefits
Types, Areas and Selection of Pentesting
Penetration Testing Scoping and Rules and rendezvous
Penetration Testing rendezvous contract and Preparation
OSINT Through World Wide Web (WWW), Website Analysis, DNS Interrogation
Automating your OSINT exertion Using Tools/Frameworks/Scripts
sociable Engineering Penetration Testing Techniques & Steps
sociable Engineering Penetration testing using E
External Network Information & Reconnaissance
Scanning, and Exploitation
Internal Network Information Reconnaissance and Scanning
Internal Network Enumeration and Vulnerability Scanning
Local and Remote System Exploitation
Firewall Security Assessment Techniques
iDs Security Assessment Techniques
Router and Switch Security Assessment Techniques
Web Application Content Discovery and Vulnerability Scanning
SQL Injection Vulnerability Penetration Testing
XSS, Parameter Tampering, fragile Cryptography, Security Misconfiguration and Client side scripting, vulnerabilities penetration techniques
Authentication, Authorization, session, Web Server Vulnerabilities Penetration Testing Information Reconnaissance
Database Enumeration & Exploitation
WLAN Penetration Testing Techniques
RFID and NFC Penetration Testing Techniques
Mobile Device Penetration Testing Techniques
loT Penetration Testing Techniques
Cloud Specific Penetration Testing Techniques and Recommendations
Cloud Specific Penetration Testing Methods
Penetration Testing Report Writing Process
Penetration Testing Reporting Formats

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ECCouncil Dumps

Identification of an unauthorized genetically modified micro organism in food enzyme via entire-genome sequencing | 412-79v9 rehearse Questions and Dumps

A meals enzyme (protease) product commercialized on the ecu market, used during this peek at, became lately suspected to be contaminated through unauthorized GMM. On the one hand, ranging from DNA extracted from the food enzyme matrix, the presence of bacterial DNA belonging to the Bacillus genus was demonstrated via PCR amplification and Sanger sequencing of the bacterial 16 S rRNA gene region8 (Fig. 1 step 1). in addition, the talents GM nature of this infection became suspected in accordance with a benign true-time PCR signal for the aminoglycoside adenyltransferase (aadD) gene conferring a resistance to each kanamycin (KanR) and neomycine (NeoR) (GenBank: M19465.1) (Fig. 1 step 1)10. The presence of the entire-length measurement of this aadD gene become as a result Tested through nested-PCR combined with Sanger sequencing, highlighting capabilities health and environmental risks linked to this food enzyme product in mild of AMR acquisition concerns9,10. nevertheless, importantly, a manageable bacterial strain changed into isolated from the proven meals enzyme product8. This bacterial stress was subjected to the identical analysis as above utilized on DNA extracted from the meals enzyme matrix. in a similar passage to the consequences observed using DNA extracted from the meals enzyme matrix, this bacterial pressure turned into shown to belong to the Bacillus genus8 as well as to heave the aadD gene (Fig. 1 step 2, desk 1, Supplementary file 1). The presence of a practicable GM Bacillus pressure within the confirmed sample turned into because of this strongly suspected, however, these outcomes were inadequate to surely prove the presence of this GMM and signify its linked genetic changes.

determine 1

Schematic representation of the workflow, composed of three main successive steps, applied on the tested food enzyme product, enabling to establish unauthorized GMM by entire-genome sequencing (WGS) and subsequently to enhance experience-particular actual-time PCR strategies. (1) DNA extracted from the FE instruction became confirmed for the presence of bacterial DNA as neatly as the presence of AMR genes commonly harboured by using GMM used to defer FE. (2) dwelling microbial strains, previous isolated from the FE education, were confirmed for the presence of bacterial DNA and subsequent selection of their genus/species as well as for the presence of AMR genes commonly harboured by passage of GMM used to supply FE. (3) The bacterial lines identified in (2) as carrying AMR genes become characterised with the aid of a WGS mode the usage of a de novo meeting evaluation so as to array the presence of a achievable unauthorized GMM within the tested FE training. With the generated sequences, actual-time PCR strategies particular to this GMM own been developed to be used by using enforcement laboratories.

table 1 Oligonucleotides used for PCR-primarily based strategies. GMM identification the usage of WGS

the usage of an Illumina MiSeq device (250 bp paired-end reads), WGS applied on DNA from the bacterial pressure isolated from the meals enzyme product (Fig. 1 step 3, Supplementary file 2) generated 714,637 paired-end uncooked reads. Following read trimming, 589,817 super reads (general Phred score of 37) own been retained to operate a de novo meeting, enabling to generate 430 contiguous sequences (contigs) of which 47 had been longer than 1,000 bases with a okay-mer coverage of at least 10x. Contig sizes ranged from 56 bp to 457,195 bp, with an N50 cost of 291,658.

On the one hand, the generated contigs offered a correspondence to the Bacillus genus, and, greater exactly, incredibly to the B. velezensis species (RefSeq: NZ_CP011937.1) as a substitute of the expected B. subtilis species that was labelled as being the producer organism of the commercialized neutral protease. This identification changed into in accordance with three observations. at the beginning, when the usage of the assembly for typing in opposition t the B. subtilis MLST schema hosted with the aid of the web-primarily based platform, an model in shape to sequence classification one hundred forty became acquired for which only a lone insulate was current in the database (PubMLST: ATCC 12321) annotated because the species B. velezensis. Secondly, a okay-mer based mostly classification of sequencing reads against an in-apartment dump of any finished genomes in the RefSeq Microbial Genomes database indicated the presence of B. velezensis (Supplementary file 3). Thirdly, this identification was proven by means of performing a read mapping evaluation to the NCBI consultant B. velezensis reference genome sequence (RefSeq: NZ_CP001937.1), with a median depth and breadth of coverage of respectively 58x and ninety four.58% (Supplementary file four). B. velezensis species is not listed via EFSA (2018) as being used within the food and feed trade to defer food and feed components, enzymes and flavourings suppositious for the eu market30. besides the fact that children, this species, for which the wild-type is harmless for human and carefully related to B. amyloliquefaciens and B. subtilis, has up to now been described as incredibly valuable for producing enzymes, including proteases, for the agro-industrial sector31,32,33,34,35,36.

however, the generated contigs own been blasted in opposition t the aadD gene, conferring KanR and NeoR, that changed into prior detected with the aid of real-time PCR as well as nested-PCR adopted by means of Sanger sequencing analysis10 (desk 1, Supplementary file 1). A contig of 349,285 bp with a okay-mer insurance of 59.434x turned into recognized as harbouring the focused AMR gene (Fig. 2, Supplementary file 5). in an exertion to identify the putative transgenic insertion, the areas flanking this AMR gene were then characterized and compared to the reference genome of B. velezensis (RefSeq: NZ_CP011937.1). within the reference genome, a Place of 2,385 bp from position 2,460,164 to 2,462,548, with >ninety nine% sequence identity, composed of a gene coding for a protease (GenPept: WP_032874795.1; RS12020 in Fig. 2) in addition to a section of a gene coding for an acetyltransferase (GenPept: WP_032874793.1; RS12025 in Fig. 2), became replaced via a fragment of 9,141 bp in the genome of the remoted bacterial strain containing the vicinity of 2,385 bp in reproduction. in view that the validated meals enzyme product turned into commercialized as a protease, the duplication of this locality can for this judgement be explained with the aid of the purpose of the manufacturers to multiply protease defer any over the creation procedure. Between these duplicated areas, a chain of four,102 bp, with a question coverage and identification of a hundred%, matching to the pUB110 shuttle vector (GenBank: M19465.1) harbouring the aadD gene (GenBank: AAA88361.1), conferring KanR and NeoR, that turned into earlier identified by true-time PCR and nested-PCR (Fig. 1), and the ble gene (RefSeq: NG_047557.1), conferring a resistance to bleomycin (BleoR) turned into characterised (Fig. 2, Supplementary file 5). This pUB110 shuttle vector, originating from Staphylococcus aureus, and the identified AMR genes were in the past pronounced as being totally used in GMM producing bacterial fermentation items within the meals and feed chain, specifically for selection of lines of interest10,37. additionally, the observed left and redress transgene flanking areas of the inserted fragment of 9,141 bp as smartly as the left and privilege transgene flanking regions of the pUB110 shuttle vector were demonstrated by PCR adopted by passage of Sanger sequencing (Supplementary data 5,6). based on any these results, the presence of a genetic change certain to a manageable GMM within the commercialized meals enzyme product changed into hence confirmed. These effects, communicated to the Belgian Federal company for the safeguard of the meals Chain, own ended in the RASFF 2019.3332 notification at the european degree.

determine 2

Schematic illustration of the identified transgenic insertion. The pUB110 shuttle vector (green) harbours the aadD gene conferring a resistance to kanamycin (KanR) (red) and the ble gene conferring a resistance to bleomycin (BleoR) (yellow). Blue rectangles depict annotated genes on the reference genome. The region indicated in orange includes a gene coding for a protease (RS12020) and section of a gene coding for an acetyltransferase (RS12025). The latter, indicated by a little dismal purple rectangle in the GM consists out of a plenary (RS12025) and interrupted (RS12025a) replica. The pink locality is enjoyable in the wild-category while this crimson vicinity is duplicated, on both sides of the pUB110 shuttle vector, in the GMM. The dismal and hatched rectangles point out the areas focused with the aid of the left (L) and privilege (R) experience-selected precise-time PCR strategies developed and validated during this examine.

regarding the bioinformatics methodology, compared to a read-mapping analysis, a de novo assembly analysis become probably the most imperative strategy to determine and symbolize an unknown and unauthorized GMM for 2 factors. originally, no reference sequence is required, representing an competencies e.g. within the existing peek at because of the unavailability of a reference sequence for the recognized GMM. This strategy is besides useful when the species identity of the GMM host is difficultly identifiable, as exemplified during this study with the Bacillus strain8. Secondly, through reconstructing a contig containing the transgenic insertion within the wild-classification B. velezensis genome, an unnatural association of sequence elements may be inferred, proposing effective evidence of the presence of a GMM. devoid of an available reference sequence for a selected GMM, a examine-mapping evaluation can't deliver this class of essential advice. indeed, handiest the presence of sequences belonging both to the pUB110 shuttle vector or to B. velezensis could then own been proven, however no hyperlink between the pUB110 shuttle vector and B. velezensis might own been dependent (Supplementary file four).

construction of event-certain precise-time PCR methods in response to WGS facts

in accordance with characterization of the transgenic insertion into B. velezensis, two event-particular actual-time PCR strategies were developed and validated, allowing to in particular goal cost- and time-successfully the unauthorized GMM organize out within the present peek at (Fig. 1 step 3). These two event-particular methods had been designed to cowl both the left or the privilege transgene flanking locality of the inserted pUB110 shuttle vector (Fig. 2; table 1; Supplementary file 5). For every precise-time PCR method, an amplicon with the expected size and sequence changed into bought (Supplementary file 7). The performance of those precise-time PCR strategies changed into then investigated.

First, the specificity of these real-time PCR methods turned into Tested the usage of, as wonderful manage, DNA from the isolated GM B. velezensis RASFF 2019.3332 pressure as well as, as snide controls, DNA from eighty-five wild-class microbial strains frequently used to provide microbial fermentation products9,10, DNA from six different wild-category B. velezensis strains, DNA from the vitamin B2-producing GM B. subtilis RASFF 2014.1249 stress, DNA from plant (Zea mays) and DNA from human. As anticipated, these adventure-particular precise-time PCR strategies presented a benign badge handiest for the advantageous control, confirming their specificity (table 2). 2d, the sensitivity of those actual-time PCR strategies became assessed using DNA from the GM B. velezensis RASFF 2019.3332 pressure at diverse estimated plenary genome copy numbers (6 × 106, 6 × 104, 6 × 102, 60, 12, 6, 1, 0.1 and nil) (table 3). For both precise-time PCR strategies, a benign signal became accompanied at as dinky as one estimated plenary genome copy, demonstrating their inordinate sensitivity. Third, the applicability of these actual-time PCR methods turned into demonstrated the exhaust of DNA from the commercialized meals enzyme product through which the GM B. velezensis RASFF 2019.3332 stress (sample n°1) turned into detected as well as a commercialized nutrition B2 feed additive product (RASFF 2014.1249) (sample n°2). As anticipated, each true-time PCR methods introduced a positive signal for the sample n°1 and a snide signal for the sample n°2 (Supplementary file 6). in line with any these effects, the two proposed event-specific true-time PCR strategies had been evaluated as specific, choice and applicable, enabling enforcement laboratories to simply target the GM B. velezensis RASFF 2019.3332 pressure in commercialized microbial fermentation products. If imperative, following to additional optimisation and validation steps, these real-time PCR strategies can be combined into a duplex assay.

desk 2 list of untamed-type microorganisms used for specificity evaluation of the real-time PCR strategies. The presence and absence of amplification are respectively symbolized via “+” and “-”. For each and every outcomes, the scan was conducted in replica. DNA from the GM Bacillus subtilis (RASFF 2014.1249) strain, ninety-one wild-type microbial traces, plant and animal own been used as negative control. DNA from the GM Bacillus velezensis (RASFF 2019.332) pressure turned into used as fanciful manage. desk three Sensitivity assessment of actual-time PCR methods. For each and every confirmed DNA concentration from the GM Bacillus velezensis RASFF 2019.3332 stress, the corresponding estimated plenary genome reproduction number is indicated. The presence and absence of amplification are respectively symbolized through “+” and “−”. For each and every result at each and every DNA concentration, the test became conducted in quadruplicate. From 25 to 0.0000025 ng, each replicate generated a benign sign. The ability of the observed Cq are indicated between brackets. From 0.00000025 to 0 ng, every replicate generated a terrible signal.

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